Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Cloning and Expression
Enzymes
MboI
Supplier Images
MboI
MboI from New England Biolabs
Be the first to write a review!
Citations:
(109)
Supplier Page
Supplier Page from
New England Biolabs for
MboI
Get Pricing
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
MboI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0147L
Quantity
2500 units
Concentration
5-25 u/µL
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
/GATC
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (109)
(1)
Examining chromatin heterogeneity through PacBio long-read sequencing of M.EcoGII methylated genomes: an m 6 A detection efficiency and calling bias correcting pipeline
bioRxiv
November 30, 2023
Allison F. Dennis, Zhuwei Xu, David J. Clark
extent of methylation at GATC sites, 0.3 μg DNA was digested with 2 U
DpnI
(
NEB
R0176S
) or 5 U
MboI
(
NEB
R0147S
) in 15 μl rCS buffer for 1 h at 37°C and analysed in a 1.2% agarose gel containing ethidium bromide.
(2)
DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in Arabidopsis
Nature Communications
November 27, 2023
Qin Li, Jincong Zhou, Shuai Li, Weifeng Zhang, Yingxue Du, Kuan Li, Yingxiang Wang, Qianwen Sun
fragmented DNA (digested by
DdeI
(
R0175S
,
NEB
),
MseI
(
R0525S
,
NEB
),
NlaIII
(
R0125S
,
NEB
) and
MboI
(
R0147S
,
NEB
) endonucleases) was used for S9.6
immunoprecipitation
. Meanwhile, the same amount of
RNase H
-treated
(3)
Lineage specific 3D genome structure in the adult human brain and neurodevelopmental changes in the chromatin interactome
Nucleic Acids Research
November 10, 2023
Samir Rahman, Pengfei Dong, Pasha Apontes, Michael B Fernando, Roman Kosoy, Kayla G Townsley, Kiran Girdhar, Jaroslav Bendl, Zhiping Shao, Ruth Misir,
et al.
Triton X-100.
Chromatin
was digested with 100 U of the 4 bp cutter
MboI
(
New England Biolabs
, Cat#
R0147L
) overnight at 37°C with shaking at 400 rpm.
MboI
was heat inactivated at 65°C for 20 min, and then nuclei
(4)
Context-dependent neocentromere activity in synthetic yeast chromosome VIII
Cell Genomics
November 8, 2023
Stephanie Lauer, Jingchuan Luo, Luciana Lazar-Stefanita, Weimin Zhang, Laura H. McCulloch, Viola Fanfani, Evgenii Lobzaev, Max A.B. Haase, Nicole Easo, Yu Zhao,
et al.
10 mL of cold 1M sorbitol and resuspended in 2 mL of 0.5% SDS at 65°C for 20 min 125 U of
MboI
(
NEB
,
R0147
) were used for overnight digestion (16 h) of the genomic DNA from fixed
yeast
cells resuspended in 3
(5)
The landscape of the histone-organized chromatin of Bdellovibrionota bacteria
bioRxiv
November 7, 2023
Georgi K. Marinov, Benjamin Doughty, Anshul Kundaje, William J. Greenleaf
CutSmart buffer
and 100 U of the
MluCI
restriction enzyme (
NEB
#R0538) plus 100 U of the
MboI
enzyme (
NEB
#
R0147
), then incubating for ≥2 hours at 37 °C in a Thermomixer at 900 rpm. The reaction was then incubated
(6)
Super-enhancer hijacking drives ectopic expression of hedgehog pathway ligands in meningiomas
Nature Communications
October 7, 2023
Mark W. Youngblood, Zeynep Erson-Omay, Chang Li, Hinda Najem, Süleyman Coșkun, Evgeniya Tyrtova, Julio D. Montejo, Danielle F. Miyagishima, Tanyeri Barak, Sayoko Nishimura,
et al.
Triton X-100 for 15 min at 37 degrees. Samples were then incubated with MboI restriction enzyme (
NEB
,
R0147
) for two hours.
MboI
was heat-inactivated for 20 min (62 degrees), followed by extension of overhands
(7)
Integrated 3D genome, epigenome and transcriptome analyses reveal transcriptional coordination of circadian rhythm in rice
Nucleic Acids Research
September 22, 2023
Ying Zhang, Guoting Chen, Li Deng, Baibai Gao, Jing Yang, Cheng Ding, Qing Zhang, Weizhi Ouyang, Minrong Guo, Wenxia Wang,
et al.
21565
) for 40 min at 25 °C. Isolated
rice
nuclei were digested with the MboI restriction enzyme (
NEB
,
R0147
). The digested
chromatin
fragments were subjected to end-repair and A-tailing using
T4 DNA polymerase
(8)
Transposable elements as tissue-specific enhancers in cancers of endodermal lineage
Nature Communications
September 1, 2023
Konsta Karttunen, Divyesh Patel, Jihan Xia, Liangru Fei, Kimmo Palin, Lauri Aaltonen, Biswajyoti Sahu
for 15 minutes at 37 °C.
Chromatin
was digested with MboI restriction enzyme (
New England Biolabs
,
R0147
) at 37 °C for 2 hours and then heat-inactivated at 62 °C for 20 minutes.
Chromatin
was incubated with
(9)
DNA-PK and the TRF2 iDDR inhibit MRN-initiated resection at leading-end telomeres
Nature Structural & Molecular Biology
September 1, 2023
Logan R. Myler, Beatrice Toia, Cara K. Vaughan, Kaori Takai, Andreea M. Matei, Peng Wu, Tanya T. Paull, Titia de Lange, Francisca Lottersberger
lauryl sarcosine) and washed five times in TE. DNA was digested overnight at 37 °C by 60 U
MboI
(no.
R0147
;
New England BioLabs
). Plugs were then washed in TE, equilibrated in 0.5× TBE and loaded on a 1% agarose/0.5×
(10)
RAD21 is the core subunit of the cohesin complex involved in directing genome organization
Genome Biology
June 28, 2023
Yuao Sun, Xin Xu, Wenxue Zhao, Yu Zhang, Keyang Chen, Yongzheng Li, Xiaotian Wang, Mengling Zhang, Boxin Xue, Wanting Yu,
et al.
in 1% formaldehyde, and suspended in Hi-C lysis buffer to which 100 U MobI restriction enzyme (
NEB
,
R0147
) was added for overnight
chromatin
digestion. Free ends were labeled with biotin and then ligated together
(11)
LEAFY homeostasis is regulated via ubiquitin-dependent degradation and sequestration in cytoplasmic condensates
iScience
June 16, 2023
Ulla Dolde, Fernando Muzzopappa, Charlotte Delesalle, Julie Neveu, Fabian Erdel, Grégory Vert
647N-31 "1,6-hexanediol",Sigma,Cat#240117 FM4-64,
Invitrogen
,Cat#
T13320
RNeasy Plant Mini Kit,
Qiagen
,Cat#
74904
M-MLV reverse transcriptase
,
Promega
,Cat#
M1701
MboI
,
NEB
,Cat#
R0147S
"
PEG
20,000",Fluka,Cat#81300 Zeba MicroSpin
desalting
column 7K MWCO,
Thermofisher
,Cat#
89877
MG132
(12)
Phenotypic dimorphism between honeybee queen and worker is regulated by complicated epigenetic modifications
iScience
April 21, 2023
Meng Jie Jin, Zi Long Wang, Zhi Hao Wu, Xu Jiang He, Yong Zhang, Qiang Huang, Li Zhen Zhang, Xiao Bo Wu, Wei Yu Yan, Zhi Jiang Zeng
commercial
assays
,Critical commercial
assays
,, 400 U MboⅠrestriction enzyme (NEB),
NEB
(USA),Cat#:
R0147L
T4 DNA ligase
(
NEB
),Enzymatics (USA),Cat#:L6030-HC-L SDS solution,Amresco (USA),Cat#:0227
proteinase K
(13)
Characterization of altered molecular mechanisms in Parkinson’s disease through cell type–resolved multiomics analyses
Science Advances
April 14, 2023
Andrew J. Lee, Changyoun Kim, Seongwan Park, Jaegeon Joo, Baekgyu Choi, Dongchan Yang, Kyoungho Jun, Junghyun Eom, Seung-Jae Lee, Sun Ju Chung,
et al.
8.0), 10 mM NaCl, and 0.2% IGEPAL CA-630 (Sigma-Aldrich, 18896) and digested with 100 U of
MboI
(
NEB
,
R0147
). Digested fragments were labeled with
biotin-14-dCTP
(
Invitrogen
,
19518018
) and proximally ligated
(14)
MYC reshapes CTCF-mediated chromatin architecture in prostate cancer
Nature Communications
March 30, 2023
Zhao Wei, Song Wang, Yaning Xu, Wenzheng Wang, Fraser Soares, Musaddeque Ahmed, Ping Su, Tingting Wang, Elias Orouji, Xin Xu,
et al.
min. The
chromatin
was digested by adding 50 μL NEB buffer 2 and 375 U MboI restriction enzyme (
NEB
,
R0147
) and incubated at 37 °C for 2 h. The MboI restriction enzyme was then inactivated by 62 °C
incubation
(15)
Umifenovir Epigenetically Targets the IL-10 Pathway in Therapy against Coxsackievirus B4 Infection
Microbiology Spectrum
February 14, 2023
Shilin Zhang, Xiao Xue, Sennan Qiao, Lin Jia, Xue Wen, Yichen Wang, Cong Wang, Hongrui Li, Jiuwei Cui
cells were lysed with lysis buffer, and the fixed DNA was sheared with
MboI
restriction endonuclease (
R0147
;
New England BioLabs
[NEB]) at 37°C for at least 2 h.
T4 DNA ligase
(
M0202
;
NEB
) was used to religate
(16)
Mapping nucleolus-associated chromatin interactions using nucleolus Hi-C reveals pattern of heterochromatin interactions
Nature Communications
January 21, 2023
Ting Peng, Yingping Hou, Haowei Meng, Yong Cao, Xiaotian Wang, Lumeng Jia, Qing Chen, Yang Zheng, Yujie Sun, Hebing Chen,
et al.
fixed with 1% formaldehyde and suspended in lysis buffer, then 100U DnpII restriction enzyme (
NEB
,
R0147
) was added. After overnight
chromatin
digestion, biotin was added to fill ends before DNA proximity
(17)
Dynamic chromatin landscape encodes programs for perinatal transition of cardiomyocytes
Cell Death Discovery
January 18, 2023
Jing Zhang, Zhaohui Ouyang, Limei Xia, Qi Wang, Feng Zheng, Kun Xu, Yuexian Xing, Ke Wei, Shaolin Shi, Chaojun Li,
et al.
formaldehyde (Sigma, F8775-25ML). Nuclei were isolated with 1% SDS, and digested using 400U
MboI
(
NEB
,
R0147
) at 37 °C overnight. After
ligation
, we sheared the
chromatin
on Sonics VCX-130 with 15 s on and 30
(18)
Cooperation of chromatin remodeling SWI/SNF complex and pioneer factor AP-1 shapes 3D enhancer landscapes
Nature Structural & Molecular Biology
January 1, 2023
Bennett K. Wolf, Yanding Zhao, Andrew McCray, William H. Hawk, Luke T. Deary, Nicholas W. Sugiarto, Ian S. LaCroix, Scott A. Gerber, Chao Cheng, Xiaofeng Wang
Buffer 2 (Cat.# B7002, New England Biolabs) and 15 μL of 25 U/μL of MboI restriction enzyme (Cat.#
R0147
,
New England Biolabs
) were added, and
chromatin
was digested at 37°C for 2 hours with rotation.
MboI
(19)
A pleiotropic hypoxia-sensitive EPAS1 enhancer is disrupted by adaptive alleles in Tibetans
Science Advances
November 25, 2022
Olivia A. Gray, Jennifer Yoo, Débora R. Sobreira, Jordan Jousma, David Witonsky, Noboru J. Sakabe, Ying-Jie Peng, Nanduri R. Prabhakar, Yun Fang, Marcelo A. Nobréga,
et al.
interacting DNA loci. Cross-linked
chromatin
was digested with Mbo I endonuclease (
New England Biolabs
,
R0147
). Subsequently, the restriction fragment overhangs were filled in, and the DNA ends were marked with
(20)
A cis-regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation
Cell Genomics
November 9, 2022
Laura K.H. Donohue, Margaret G. Guo, Yang Zhao, Namyoung Jung, Rose T. Bussat, Daniel S. Kim, Poornima H. Neela, Laura N. Kellman, Omar S. Garcia, Robin M. Meyers,
et al.
was digested using 50 uL
NEBuffer 2
(
NEB
,
B7002S
) and 8 uL of 375 U of MboI restriction enzyme (
NEB
,
R0147
) with rotation at 37C for 15 min. Digested
chromatin
was spun down for 5 min at 2500 rcf, supernatant
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Be the First to Write a Review!
MboI from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Back To Top